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Prep SFC 50

The Prep SFC 50 from Sepiatec is a compact and easy-to-use chromatography system for preparative separations on small columns by means of supercritical fluid chromatography (SFC). The system offers the comprehensive benefits of SFC technology, such as faster separations, lower costs due to reduced consumption of organic solvents, as well as being more environmentally friendly at increased safety.

The Prep SFC 50 is equipped with two 400 bar high-pressure pumps with 30 ml pump heads. The total flow – at a modifier portion of 40 % – amounts to 50 ml/min. This way, chromatography columns with inner diameters ranging from 4 to 16 mm, and a length of up to 250 mm can be used.

The column oven, containing the chromatography columns together with the injection valve and the UV flow cell, is heatable up to 70° C so that a uniform temperature is ensured. The Prep SFC 50 system is equipped with 9 gas-liquid-separators (1 for waste) for precise separation of chiral compounds.

As fractions are collected in unpressurized state, not only safety is increased, but also handling is simplified, and limitations with respect to the size of fraction volumes do not exist. This option proves to be extremely convenient when working with stack injections.

As the dimensions of the Prep SFC 50 together with the integrated system controller and 15.6” touch screen amount to only 56 x 60 x 88 cm (W x D x H) this system requires a minimum of space in the laboratory. A separate computer or monitor is not necessary.

The equipment of the Prep SFC 50 system includes

  • a high pressure pump for CO2
  • a high pressure pump for modifiers
  • an adjustable back pressure regulator
  • a syringe pump for sample injection
  • a UV detector
  • an integrated system controller
  • a 15.6“ touch screen
  • USB connectors, Ethernet and HDMI connector
  • the Prep SFC control software

The equipment can be expanded with optional accessories

  • Add-on pump  for separations with low modifier portions
  • Diode array detector (PDA)
  • ELSD detector
  • Mass spectrometer

Prep SFC 50 system with mass spectrometer

The expression CMS from Advion is a very compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of Prep SFC 50 and expression CMS, allows users to perform mass-directed-, mass-confirmed- and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactive targets can be fractionated.

The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required.

All the modules needed to connect the expression CMS to the Prep SFC 50 system, such as a makeup flow pump and a split valve, are included. The expression CMS mass spectrometer is operated and the data obtained are displayed using the Prep SFC control software. The expression CMS software allows users to perform a wide range of assessments. The compact size of the Prep SFC 50 and the expression CMS means that they can be installed together in a standard laboratory fume hood.

Prep SFC 50 system with mass spectrometer

Prep SFC 50 software

The Prep SFC 50 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens.

The Prep SFC control software includes a sequence editor for inputting separation parameters for different samples, functions such as stack injection with automatic suggestions for stack times, multiple options for fractionation and on-the-fly editing for changing parameters during the course of a run.

Prep SFC 50 software
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Prep SFC 50 system with mass spectrometer

Prep SFC 50 system with mass spectrometer

The expression CMS from Advion is a very compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of Prep SFC 50 and expression CMS, allows users to perform mass-directed-, mass-confirmed- and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactive targets can be fractionated.

The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required.

All the modules needed to connect the expression CMS to the Prep SFC 50 system, such as a makeup flow pump and a split valve, are included. The expression CMS mass spectrometer is operated and the data obtained are displayed using the Prep SFC control software. The expression CMS software allows users to perform a wide range of assessments. The compact size of the Prep SFC 50 and the expression CMS means that they can be installed together in a standard laboratory fume hood.

Prep SFC 50 software

Prep SFC 50 software

The Prep SFC 50 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens.

The Prep SFC control software includes a sequence editor for inputting separation parameters for different samples, functions such as stack injection with automatic suggestions for stack times, multiple options for fractionation and on-the-fly editing for changing parameters during the course of a run.

 

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