SFC-250
Preparative scale separation – compact design
Sepiatec’s new SFC-250 system is a compact benchtop unit designed for preparative separation using supercritical fluid chromatography.
Measuring just 72 x 60 x 88 cm (W x D x H) the system offers all advantages of SFC technology such as fast separation, reduced consumption of organic solvents, lower costs, improved environmental compatibility and increased safety.
The SFC-250 system uses two high-pressure pumps fitted with 150 ml pump heads. Total flow rates of up to 250 ml of supercritical fluid per minute can be achieved at a modifier portion of 40%. This allows the operator to use separation columns with internal diameters of 15 to 30 mm and lengths of 250 mm.
Due to its compact design the footprint of the SFC-250 system is comparatively small and precious laboratory space can be saved. The clearly arranged assembly of the SFC-250 system allows easy access to all of its modules. The fractionation module includes a selector valve and 9 gas-liquid separators (1 for waste). It is designed for 8 fraction bottles with a volume of 1 litre each. Due to the flexible outlets even bigger sample containers with volumes up to several litres can be used.
A system controller with current performance characteristics is built into the device. There is no need for a separate computer or monitor, and no additional bench space is required.
The very user-friendly Prep SFC control software runs the entire device and contains functions such as stack injection, peak detection and on-the-fly editing. Data is entered via the 15.6‘‘ touch screen, which is placed ergonomically on the upper front of the SFC-250 system.
The equipment of the SFC-250 system includes
- a high pressure pump for CO2
- a high pressure pump for modifiers
- an add-on pump for separations with low modifier portions
- an adjustable back pressure regulator
- a syringe pump for sample injection
- a diode array detector (PDA)
- an integrated system controller
- a 15.6“ touch screen
- the Prep SFC Control software
The equipment can be expanded with optional accessories
- ELSD detector
- Mass spectrometer
SFC-250 system with mass spectrometer
The expression CMS from Advion is a very compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of SFC-250 and expression CMS, allows users to perform mass-directed-, mass-confirmed- and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactive targets can be fractionated.
The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required. The acquisition rate is 5,000 m/z units sec-1 and the resolution is 0.5 – 0.7 m/z units (FWHM) at 1,000 m/z units sec-1 throughout the acquisition range. All the modules needed to connect the expression CMS to the SFC-250 system, such as a makeup flow pump and a split valve, are included.
The expression CMS mass spectrometer is operated and the data obtained are displayed using the SFC-250 system software. The expression CMS software allows users to perform a wide range of assessments. The compact size of the SFC-250 and the expression CMS means that they can be installed together in a standard laboratory fume hood.
SFC-250 software
The SFC-250 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens.
The Prep SFC Control software includes a sequence editor for inputting separation parameters for different samples, functions such as stack injection with automatic suggestions for stack times, multiple options for fractionation and on-the-fly editing for changing parameters during the course of a run.
SFC-250 system with mass spectrometer
The expression CMS from Advion is a very compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of SFC-250 and expression CMS, allows users to perform mass-directed-, mass-confirmed- and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactive targets can be fractionated.
The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required. The acquisition rate is 5,000 m/z units sec-1 and the resolution is 0.5 – 0.7 m/z units (FWHM) at 1,000 m/z units sec-1 throughout the acquisition range. All the modules needed to connect the expression CMS to the SFC-250 system, such as a makeup flow pump and a split valve, are included.
The expression CMS mass spectrometer is operated and the data obtained are displayed using the SFC-250 system software. The expression CMS software allows users to perform a wide range of assessments. The compact size of the SFC-250 and the expression CMS means that they can be installed together in a standard laboratory fume hood.
SFC-250 software
The SFC-250 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens.
The Prep SFC Control software includes a sequence editor for inputting separation parameters for different samples, functions such as stack injection with automatic suggestions for stack times, multiple options for fractionation and on-the-fly editing for changing parameters during the course of a run.