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Prep SFC M5-50

Sepiatec’s Prep SFC M5-50 system is a preparative system for separating chiral and achiral samples using supercritical fluid chromatography.

The Prep SFC M5-50 system consists of the main module and the collector module. Both modules form a functional unit that can be configured in a variety of ways with different equipment. The main module can be equipped with up to three high pressure pumps, a CO2 pump and a modifier pump with 30 ml pump heads, 400 bar, and an optional add-on pump with 30 ml pump head, 400 bar, for separations with low modifier portions.

The integrated auto sampler can accommodate two standard or two deep well microtiter plates with 24 wells and a volume of 10 ml each or racks with up to 60 sample vials with a volume of 1.5 ml each. The heatable column oven is suitable for up to 10 separation columns with internal diameter of 4 to 16 mm and a length of 250 mm.

Standard equipment in the main module is a UV detector for measuring in the range of 190 – 500 nm. Other detectors, such as a DAD or an ELSD, can also be connected. An integrated system controller and a 15.6” TFT touch screen are used to input all data for sample separations. No additional computer is needed, reducing the space required for the Prep SFC M5 system to a width of just 92 cm.

The post heater and the back pressure regulator are integrated within the collector module. The continuously operating gas-liquid separator is located at the front of the collector module; it removes residual CO2 from the isolated compounds.

The Prep SFC M5-50 system can be fitted with a carousel fraction collector which can accommodate collection vessels with volumes ranging from 10 to 500 ml. Alternatively, an XY-fraction collector for vials with volumes of 10 or 60 ml is available. A total of up to 195 fractions can be collected.

Prep SFC M5-50 system with mass spectrometer

For mass-directed fractionation the Prep SFC M5 system can be combined with Advion’s expression CMS.

The expression CMS is a compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of the Prep SFC M5 and the expression CMS, allows users to perform mass-directed, mass-confirmed and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactice targets can be fractionated.

The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required.

The expression CMS mass spectrometer is operated and the data obtained are displayed using the Prep SFC control software.

Prep SFC M5-50 system with mass 
spectrometer

Prep SFC M5-50 software

The Prep SFC M5 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens.
The software includes a sequence editor for inputting separation parameters for different samples, functions such as stack injection with automatic suggestions for stack times, multiple options for fractionation and on-the-fly editing for changing parameters during the course of a run.

An additional monitor at a separate work site can also be connected.

Prep SFC M5-50 software
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Prep SFC M5-50 system with mass 
spectrometer

Prep SFC M5-50 system with mass spectrometer

For mass-directed fractionation the Prep SFC M5 system can be combined with Advion’s expression CMS.

The expression CMS is a compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of the Prep SFC M5 and the expression CMS, allows users to perform mass-directed, mass-confirmed and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactice targets can be fractionated.

The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required.

The expression CMS mass spectrometer is operated and the data obtained are displayed using the Prep SFC control software.

Prep SFC M5-50 software

Prep SFC M5-50 software

The Prep SFC M5 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens.
The software includes a sequence editor for inputting separation parameters for different samples, functions such as stack injection with automatic suggestions for stack times, multiple options for fractionation and on-the-fly editing for changing parameters during the course of a run.

An additional monitor at a separate work site can also be connected.

 

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