SEPBOX 2D-5000


Today, HPLC is the standard technology used for separating compounds from natural resources.
Sepiatec’s unique Sepbox systems allow samples to be processed automatically, and significantly accelerate the purification process. Results which usually take weeks or months with conventional methods can be obtained in just a few days – or even one day – with a Sepbox system.

Combination of HPLC and SPE

The Sepbox concept is based on a patented combination of HPLC and SPE (Solid Phase Extraction) – reliable and proven techniques that provide a universal platform suitable for processing large sample numbers.

Up to 576 fractions with a very high yield of pure compounds can be efficiently obtained for subsequent High Throughput Screening. Using two-dimensional separation, the recovery rate for both polar and non-polar substances is usually above 90 per cent.

Using an automated and highly reproducible process, one extract can be completely separated per day. The pure individual compounds are soluted in suitable solvents and can be collected in vials (Sepbox 2D-5000 and Sepbox 2D-2000) or microtiter plates (Sepbox 2D-250).

Up to 5,000 milligrams of extract

The Sepbox 2D-5000 is designed for a large workload of up to 5,000 milligrams of extract made from plants, marine organisms, microbiological or animal material.

The automated 2D chromatography provides 576 fractions in 24 hours. The fraction collector is equipped with 12 removable racks, each containing 48 vials with a maximum capacity of 60 millilitres. The Sepbox 2D-5000 is also equipped with software for method development, process control, data acquisition and data analysis.

The Sepbox 2D-5000 provides many individual chemical entities in quantities which can be easily used to elucidate structures, screen different targets and build a diverse library.


Isolation of compounds with a speed and accuracy unachievable with conventional  chromatography methods
On average 20 to 30 per cent pure fractions (ELSD) per run
Isolation of large quantities possible
Reproducible separations
Cost advantages due to automation

Purity of Sepbox fractions

On average after one Sepbox run, up to 20 per cent of all fractions from plant extracts are 90 to 99 per cent pure (ELSD*), and from extracts made of marine organisms, up to 30 per cent are 90 to 99 per cent pure (ELSD).

As well as these highly purified compounds, the user will usually find a large number of fractions with compounds whose purity levels are between 50 and 90 per cent in ELSD detection. Results vary depending on the extract and the examined organism.

* The purity levels quoted here were calculated using the 100 per cent method with ELSD detection, as UV detection does not provide reliable purity levels due to different absorption coefficients.

Modes of operation Medium to non-polar, Polar Setup (optional)
Sample size Max. 5,000 mg of extract
Pumps 4 preparative HPLC pumps
Columns 1 injection column, 1 main separation column, 3 second separation columns (6 with Polar Setup), 28 trap columns
Detection 2 UV detectors, 1 ELSD detector
Fraction collector Fraction collector with 12 racks, 48 vials each, max. capacity 60 ml/vial, max. 576 fractions
Modes of collection Time and peak based, baseline collect (optional)
Valves 67 pneumatically actuated valves
Software Sepbox Control Software
Dimensions Sepbox: 170 x 94 x 193 cm (W x D x H)
Fraction Collector: 140 x 76 x 168 cm (W x D x H)



Sepbox 2D-5000 rear view


Detail Sepbox columns

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